ISG15 (menselijk) (Rhodamine 110)

Source: E. coli , MW ~18kDa , ISG15 (Interferon Stimulated Gene, 15kDa) is a ubiquitin-like modifier which initiates innate immune response by activating RIG-I signaling, stimulating NK-cell proliferation, inhibiting viral budding and acting as an IFNÎ3-inducing cytokine. ISG15 contains two tandem ubiquitin homology domains and is cross-reactive with α-Ubiquitin antibodies. Conjugation of ISG15 to a substrate protein occurs through a ubiquitin like cascade via an E1 (UBE1L), E2 (UbcH8/UBE2L6) and E3 (not yet discovered.) Deconjugation normally occurs via UBP43 (USP18), however there are several viral proteases that are able to hydrolyze ISG15 conjugates in order to evade immune response. These include the OTU-containing protease of Crimean-Congo Hemorrhagic Fever nairovirus and the Papain-Like Protease (PLPro) of the SARS coronavirus.

ISG15 Ubiquitin-like Modifier; G1P2; IFI15; IP17; UCRP

P05161

Human

>=97% (LCMS)

Liquid. In 50mM MES pH 6.0, 100mM sodium chloride, 10% glycerol.

Short Term Storage -80°C Long Term Storage -80°C Handling Advice Aliquot to avoid freeze/thaw cycles. Use/Stability Stable for at least 1 year after receipt when stored at -80°C.

Protein-based substrate. Typical working concentration range is 50-500nM. Hydrolysis of the conjugate results in fluorescence observable by excitation at 485nm and emission at 535nm.

Human ISG15 (aa1-157) (Accession Nr. P05161) conjugated at the C-terminus to a quenched Rhodamine 110 dye.