Islet 1 Antilichaam / ISL1

Insulin gene enhancer protein ISL-1 is a protein that in humans is encoded by the isl1 gene. This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Western blot: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, ELISA: 0.1-0.5 µg/mL

P61371

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids D118-E161) was used as the immunogen for the Islet 1 antibody.

WB, IF, IHC-P, ELISA

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Islet 1 antibody is available for research use only.

After reconstitution, the Islet 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Islet 1 antibody should be determined by the researcher.

Nuclear

Immunofluorescent staining of FFPE rat brain tissue with Islet 1 antibody (green) . HIER: steam section in pH8 EDTA buffer for 20 min.