JNK1 / JNK2 (fosfo Thr183 / Tyr185) Antilichaam

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4) . /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it./Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity

5599;5601;5602

P45983P45984P53779

NP_002741.1; NP_001128516.1; NP_002744.1

Human, Mouse, Rat

JNK1/JNK2 (phospho-Thr183/Tyr185) antibody was raised against a peptide sequence around phosphorylation site of Thr183/Tyr185 (M-M-T (p) -P-Y (p) - V - V) derived from Human JNK1/JNK2.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

46, 54 kDa

This antibody detects endogenous level of JNK1/JNK2 only when phosphorylated at Thr183/Tyr185.

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

MAPK8 MAPK9 MAPK10

Mitogen-activated protein kinase 8 / mitogen-activated protein kinase 9 / mitogen-activated protein kinase 10

Homo sapiens

Davis, R. J. (1999) Biochem Soc Symp 64, 1-12. Ichijo, H. (1999) Oncogene 18, 6087-93. Kyriakis, J. M. and Avruch, J. (2001) Physiol Rev 81, 807-69.

JNK, JNK1, PRKM8, SAPK1, JNK-46, JNK1A2, SAPK1c, JNK21B1/2, FLT, FRT, VEGFR1, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1

WB, IF

4506095 / 205277412 / 4506081

Liquid