Jurkat Lysate

Jurkat cells are an immortalized line of human T lymphocyte cells take from the peripheral blood of a 14-year-old boy with T cell leukemia, in 1970s. Jurkat cells are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV. Jurkat cells are also useful in science because of their ability to produce interleukin 2. Their primary use, however, is to determine the mechanism of differential susceptibility of cancers to drugs and radiation.

Human

WB

2 mg/mL (determined by BCA assay)

Jurkat, ATGL0003-10 µg, ATGL0003-20 µg, ATGL0003-50 µg, ATGL0003-100 µg, ATGL0003-250 µg, ATGL0003-500 µg, ATGL0003-1 mg, ATGL0003-10, ATGL0003-20, ATGL0003-50, ATGL0003-100, ATGL0003-250, ATGL0003-500, ATGL0003-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : Jurkat Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody