K27-linkage Specifiek Ubiquitin-antilichaam / UBB / UBC

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200

P0CG47, P0CG48

Human, Mouse, Rat

A synthesized peptide derived from human K27-linkage Specific Ubiquitin was used as the immunogen for the K27-linkage Specific Ubiquitin antibody.

31U80

WB, IHC, ICC, IF

Affinity chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This K27-linkage Specific Ubiquitin antibody is available for research use only.

Store the K27-linkage Specific Ubiquitin antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the K27-linkage Specific Ubiquitin antibody should be determined by the researcher.

Western blot analysis of K27-linkage Specific Ubiquitin expression in human HeLa cell lysate using K27-linkage Specific Ubiquitin antibody. A strong band near ~20 kDa in HeLa lysate is detected, corresponding to K27-linked di-ubiquitin. Although the UBB and UBC precursors have predicted masses of ~26 kDa and ~77 kDa, they are rapidly processed into monomeric and short polyubiquitin chains in cells; K27-linked Ub2 species are therefore the predominant form detected under standard conditions.