Keratine 20 Antilichaam / Cytokeratine 20 / CK20

This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20) . KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.

Western blot: 2-4 µg/mL

P35900

Human

A recombinant partial protein from human KRT20 protein (within amino acids 196-323) was used as the immunogen for the Cytokeratin 20 antibody.

KRT20/3224R

WB

Protein A affinity

Purified

This Cytokeratin 20 antibody is available for research use only.

Aliquot the Cytokeratin 20 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the Cytokeratin 20 antibody should be determined by the researcher.

Cytoplasm

Western blot testing of human HT29 cell lysate with Keratin 20 antibody (clone KRT20/3224R) . Predicted molecular weight ~46 kDa.