KG-1 Lysate

KG-1 cell line derived from the bone marrow aspirate of a 59 year old male Caucasian with erythroleukemia that became acute myelogenous leukaemia. KG-1 cells resemble acute myelogenous leukaemia showing considerable pleomorphism with a predominance of myeloblasts and promyelocytes. A small percentage of the cells are mature granulocytes with the occasional presence of macrophages and eosinophils. They do not express surface Ig or EBV antigens and lack specific markers for lymphocytic cells.

Human

WB

2 mg/mL (determined by BCA assay)

KG1, ATGL0036-10 µg, ATGL0036-20 µg, ATGL0036-50 µg, ATGL0036-100 µg, ATGL0036-250 µg, ATGL0036-500 µg, ATGL0036-1 mg, ATGL0036-10, ATGL0036-20, ATGL0036-50, ATGL0036-100, ATGL0036-250, ATGL0036-500, ATGL0036-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : KG-1 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody