KSHVORF45 Antilichaam [2D4A5]

Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma- (2) -herpesvirus subfamily and has been closely linked to the Kaposi’s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman’s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions.

4961474

F5HDE4

F5HDE4

Virus

Ni-NTA purified recombinant human KSHV ORF45 expressed in E. Coli strain BL21 (DE3) .

2D4A5

Unconjugated

Primary Antibodies

Infectious Disease

Batch dependent

Ascitic fluid containing 0.03% sodium azide.

None

Blue Ice

To KSHV ORF45 monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG1

ORF45

Virion phosphoprotein

Human herpesvirus 8

Chang Y. et al. 1994. Science. 266:1865-1869.

Zhu FX. et al. 2002. PNAS. 99: 5573-5578.

Zhu FX. et al. J Virol. 2003. April (77) : 4221-4230.

Protein ORF45

ELISA, WB

550540839