L-929 Lysaat

L-929 mouse fibroblast cells line established by W. R Earle in 1940. This strain was derived from normal subcutaneous areolar and adipose tissue of a 100 day old male C3H/An mouse. Cells are APRT+ and HPRT+. L929 cells are used in studies of TNF-alpha, TNF-beta bioassays and cytokine assays.

Mouse

WB

2 mg/mL (determined by BCA assay)

L929, ATGL0031-10 µg, ATGL0031-20 µg, ATGL0031-50 µg, ATGL0031-100 µg, ATGL0031-250 µg, ATGL0031-500 µg, ATGL0031-1 mg, ATGL0031-10, ATGL0031-20, ATGL0031-50, ATGL0031-100, ATGL0031-250, ATGL0031-500, ATGL0031-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Mouse

All lanes : L-929 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody