LAG-1 Recombinant eiwit

Source : Escherichia Coli. LAG-1 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 69 amino acids and having a molecular mass of 7.8kDa.The CCL4L1 is purified by proprietary chromatographic techniques. CCL4L1 (C-C motif chemokine 4-like) is a member of to intercrine beta (chemokine CC) family. The CCL4L1 protein is similar to CCL4 which inhibits HIV replication in peripheral blood monocytes which express CCR5.

C-C motif chemokine 4-like||Lymphocyte activation gene 1 protein||LAG-1||Macrophage inflammatory protein 1-beta||MIP-1-beta||Monocyte adherence-induced protein 5-alpha||Small-inducible cytokine A4-like||CCL4L1||CCL4L||LAG1||SCYA4L1||CCL4L2||SCYA4L2||

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

LAG-1 was lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.

Lyophilized LAG-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL4L1 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

It is recommended to reconstitute the lyophilized LAG-1 in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. The ED50 as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 ƒâ€” 105 IU/mg.

APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN.