LAG-3 Enkelvoudig Domin-antilichaam [2A11]

The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2) . It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4) . LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.

3902

P18627

NP_002277

Human

LAG3 antibody was raised against a recombinant protein corresponding to amino acids 23-434 of human LAG3.

2A11

Unconjugated

Primary Antibodies

Immunology

LAG3 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.

1 mg/mL

LAG-3 Antibody is supplied in PBS.

None

Dry Ice

LAG3 antibody should be stored in working aliquots at -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

SdAb

LAG-3 antibody can be used for ELISA starting at 0.1 μ g/mL. For immunohistochemistry starting at 1 μ g/mL. For immunofluorescence start at 5 μ g/mL. For flow cytometry at 1 μ g/ml.

LAG3

Lymphocyte activating 3

Homo sapiens

Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216–21.

Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619–22.

Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885–91.

Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970–9.

LAG3 Antibody: lymphocyte activating 3, LAG-3, CD223

ELISA, IHC-P, IF, Flow

167614500

Liquid