Laminine alfa 4 (LAMA4) (NM 001105206) Humaan Recombinant Proteïne

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the alpha chain isoform laminin, alpha 4. The domain structure of alpha 4 is similar to that of alpha 3, both of which resemble truncated versions of alpha 1 and alpha 2, in that approximately 1,200 residues at the N-terminus (domains IV, V and VI) have been lost. Laminin, alpha 4 contains the C-terminal G domain which distinguishes all alpha chains from the beta and gamma chains. The RNA analysis from adult and fetal tissues revealed developmental regulation of expression, however, the exact function of laminin, alpha 4 is not known. Tissue-specific utilization of alternative polyA-signal has been described in literature. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011]

Purified recombinant protein of Human laminin, alpha 4 (LAMA4), transcript variant 1, Ala25-Thr270, with N-terminal His tag, expressed in E. coli, 50ug

CMD1JJ; LAMA3; LAMA4*-1

3910

Q16363, A0A0A0MQS9

NM_001105206

6q21

E. coli

N-His

ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer

ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer

>50 ug/mL as determined by microplate BCA method

> 80% as determined by SDS-PAGE and Coomassie blue staining

Liquid

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol.

Druggable Genome, Secreted Protein

26.1 kDa

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

LAMA4

Human

NP_001098676

https://www.ncbi.nlm.nih.gov/gene/3910

https://www.uniprot.org/uniprot/Q16363

https://www.ncbi.nlm.nih.gov/nuccore/NM_001105206

https://www.ncbi.nlm.nih.gov/nuccore/NP_001098676