Lba Cas12a (Cpf1) Nuclease

Lba Cas12a nuclease is derived from Lachnospiraceae bacterium ND2006 and is a site-specific DNA endonuclease mediated by a single-guide RNA (gRNA) with a length of 41-44 nucleotides. In the presence of a PAM (TTTN) sequence on the target double-stranded DNA, LbCas12a specifically cleaves the target dsDNA, resulting in double-strand breaks and the generation of sticky ends. In contrast, the specific cleavage of single-stranded DNA targets by LbCas12a does not require a PAM sequence. Lba Cas12a contains simian virus 40 (SV40) T antigen nuclear localization sequences (NLS) at both the N-terminus and C-terminus. When Cas12a is expressed with these NLS sequences, the Cas12a-RNP complex can rapidly localize to the nucleus upon cellular entry. Additionally, both double-stranded and single-stranded DNA targets can activate the trans-cleavage activity of LbCas12a. Specifically, upon formation of a ternary complex among the LbCas12a enzyme, crRNA, and target DNA, the enzyme becomes activated and exhibits non-specific trans-cleavage activity, degrading any single-stranded DNA (ssDNA) present in the system. Consequently, LbCas12a can not only be used for specific cleavage of dsDNA in vitro but also enables rapid detection of target nucleic acids.

CRISPR-associated endonuclease Cas12a, CRISPR-associated endonuclease Cpf1, Lba Cas12a (Cpf1), LbaCas12a, Cpf1, LbCas12a

Recombinant

Genome Editing, DNA Detection, CRISPR Diagnostics

Store at -20°C for twelve months from the date of receipt.

Please contact with the lab for this information.

>90% as determined by SDS-PAGE.

Liquid

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

For research use only.

Lachnospiraceae bacterium ND2006