LCTL Antilichaam/Lactase-achtig eiwit

Lactase-like is a protein that in humans is encoded by the LCTL gene. This gene encodes a member of family 1 glycosidases. Glycosidases are enzymes that hydrolyze glycosidic bonds and are classified into families based on primary amino acid sequence. Most members of family 1 have two conserved glutamic acid residues, which are required for enzymatic activity. The mouse ortholog of this protein has been characterized and has a domain structure of an N-terminal signal peptide, glycosidase domain, transmembrane domain, and a short cytoplasmic tail. It lacks one of the conserved glutamic acid residues important for catalysis, and its function remains to be determined (PMID: 12084582) . Alternative splicing results in multiple transcript variants.

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q6UWM7

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids D67-Q537) was used as the immunogen for the LCTL antibody.

WB, FACS, ELISA

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This LCTL antibody is available for research use only.

After reconstitution, the LCTL antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the LCTL antibody should be determined by the researcher.

Flow cytometry testing of fixed and permeabilized human HepG2 cells with LCTL antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= LCTL antibody.