LDHD Antilichaam / D-lactate dehydrogenase

Two naturally occurring forms of lactate dehydrogenase with similar but unique substrate specificities have been isolated in lower organisms including invertebrates, fungi, and prokaryotes. These dehydrogenase enzymes are L-lactate dehydrogenase and D-lactate dehydrogenase (LDHD) that are specific to the L and D isomers of lactate, respectively (PMID: 12127981) . In lactic acid bacteria, LDHD plays a key role in anaerobic energy metabolism (PMID: 497162) . Despite the identification of D-lactate and other D-2-hydroxyacids in prokaryotes, and the obvious connections and similarities to vertebrate metabolic pathways, very few mammalian D-2-hydroxyacid dehydrogenases have been found. LDHD has 2 isoforms with the molecular weight of 52 and 54kDa, and can be detected as 45-54 kDa.

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q86WU2

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids R56-Q498) was used as the immunogen for the LDHD antibody.

WB, FACS, ELISA

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This LDHD antibody is available for research use only.

After reconstitution, the LDHD antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the LDHD antibody should be determined by the researcher.

Flow cytometry testing of fixed and permeabilized human K562 cells with LDHD antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= LDHD antibody.