Leptine, gepegyleerd

Mouse Leptin is as key regulator of energy balance and body weight control. Following its release into the bloodstream, it binds to the leptin receptor (LEPR), a membrane-bound receptor widely expressed in both central and peripheral tissues. This interaction initiates several major signaling pathways, which collectively regulate energy homeostasis, appetite, metabolism, and other physiological functions. Mutations or defects in the lep gene can lead to severe obesity and the development of type II diabetes. Pegylated recombinant mouse leptin is a polypeptide chain containing 146 amino acid residues with an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus. The expected molecular mass is ~ 35.6 kDa as determined by MS. However, due to enlarged hydrodynamic volume, the recombinant leptin runs on the SDS-Page as a 48 kDa protein. After subcutaneous injection, the half-life in circulation of the recombinant mouse leptin was tested to be over 20 hours.

Lep; ob; obese

16846

P41160

NP_032519.1

NM_008493.3

Mouse

Mouse

PEG

AVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC

It is recommended to reconstitute the lyophilized recombinant mouse pegylated leptin in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions.

< 0.1 ng/µg of protein (< 1EU/µg)

> 98.0% as determined by Gel filtration and SDS-PAGE gel.

Recombinant mouse pegylated leptin is capable of stimulatng proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its in vitro activity is only slightly lower than the non-pegylated recombinant mouse leptin but in vivo it has profound weight reducing effect (as compared to the non-pegylated recombinant mouse leptin), resulting mainly from reduced food intake.

146

Lyophilized

Water

35.6 KkDa

E. coli