Leptinereceptor / LEPR / CD295 eiwit (His & hFc-tag)

Leptin Receptor or CD295 belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight) and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Leptin Receptor/CD295 is transmembrane catalytic receptors found on NPY/AgRP and alpha-MSH/CART neurons in hypothalamic nuclei. Leptin receptors (Ob-Rs) are coded for by one human gene that produces six different isoforms; Ob-Ra - Ob-Rf. Ob-Rs exist as constitutive dimers at physiological expression levels. Only the Ob-Rb isoform can transduce intracellular signals and does so through activation of the JAK2/STAT3, PI 3-K, and MAPK signaling cascades. Activation of Ob-Rs mediates transcriptional regulation of the hypothalamic melanocortin pathway and downregulates endocannabinoid expression. Leptin acts via leptin receptors. Leptin resistance has been proposed as a pathophysiological mechanism of obesity. In obese individuals, Ob-Ra (which is involved in the active transport of leptin across the blood-brain barrier) expression is downregulated and the individual may be unresponsive to leptin signals. Ob-R antagonists are of great interest in the development of pharmacological treatments for obesity. Mutations in the Leptin Receptor/CD295 have been associated with obesity and pituitary dysfunction.

CD295 Protein; DB Protein; LEP-R Protein; LEPRD Protein; OB-R Protein; OBR Protein

NP_002294.2

Human

Human

His-/hFc-Tag

See Datasheet send with the product

< 1.0 EU per μg of the protein as determined by the LAL method

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Measured by its binding ability in a functional ELISA . Immobilized human Leptin at 1.25 μg/mL (100 μL/well) can bind human Leptin receptor with a linear range of 0.032-4.0 μg/mL.

1065

Lyophilized

PBS, pH 7.4

See Datasheet

160 kDa (glycosylated)

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

HEK 293 cells