LGALS8 Recombinant eiwit

Source : Escherichia Coli. Galectin-8 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 317 amino acids and having a molecular mass of 35.8kDa.The LGALS8 is purified by proprietary chromatographic techniques. Galectins are a family of animal lectins with an affinity for beta-galactosides. This family has at least 14 identified members. Galectins share similarities in the CRD (the carbohydrate recognition domain) . Galectins are synthesized as cytosolic proteins. Though localized principally in the cytoplasm and lacking a classical signal peptide, galectins can also be stimulated to secretion by non-classical pathways or alternatively targeted to the nucleus. Galectins are involved in modulating cell-cell and cell-matrix interactions. Galectin-8 is a tandem-repeat-type member of the galectin family, consisting of 2 CRDs attached by a linker peptide. Galectin-8 is greatly expressed in lung carcinomas, a number of forms of prostate carcinomas, in addition to other tumor cells. Galectin-8 attaches to a subset of cell surface integrins to modulate ECM-integrin interactions. Once immobilized, Galectin-8 promotes cell adhesion by ligation and clustering of cell surface integrin receptors. On the other hand, as a soluble ligand, Galectin-8 can inhibit cell adhesion.

Galectin-8||Gal-8||Po66 carbohydrate-binding protein||Po66-CBP||Prostate carcinoma tumor antigen 1||PCTA-1||LGALS8.

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

LGALS8 was lyophilized from a concentrated (1mg/mL) solution in 20mM PBS, pH 7.4.

Lyophilized LGALS8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Galectin-8 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

It is recommended to reconstitute the lyophilized Galectin-8 in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. The ED50 of Galectin-8 as determined by its ability to agglutinate human red blood cells is 0.8~4 μg/ml.

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Met-Leu-Ser-Leu.