LIM Kinase 1 (LIMK1) Mouse Monoclonal Antibody [Kloon ID: LBI8C8]

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Carrier-free (BSA/glycerol-free) LIMK1 mouse monoclonal antibody, clone LBI8C8

LIMK; LIMK-1

Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1, mRNA.

3984

P53667

Unconjugated

Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton

Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton

WB 1:1000, IHC 1:150, IF 1:100, FLOW 1:100

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Druggable Genome, Protein Kinase

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

LIMK1

https://www.ncbi.nlm.nih.gov/gene/?term=3984

https://www.uniprot.org/uniprot/P53667