LIN28B Antilichaam

Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

389421

Q6ZN17

NP_001004317.1

Human

LIN28B antibody was raised against a peptide sequence around aa.242~246 (P-S-V-Q-K) derived from Human LIN28B.

Unconjugated

Primary Antibodies

Stem Cell

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

None

Blue Ice

Store antibody at -20˚ C for up to one year.

21 32 kDa

This antibody detects endogenous levels of total LIN28B protein.

Western Blot: 1:500~1:1000

LIN28B

Lin-28 homolog B (C. elegans)

Homo sapiens

"Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma. "Guo Y., Chen Y., Ito H., et al. Gene 384:51-61 (2006) "Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA. "Heo I., Joo C., Cho J., Ha M., Han J., Kim V. N. Mol. Cell 32:276-284 (2008) "TUT4 in concert with Lin28 suppresses MicroRNA biogenesis through pre-microRNA uridylation. "Heo I., Joo C., Kim Y. -K., Kim V. N., et al. Cell 138:696-708 (2009)

CSDD2, CTNNB, OK/SW-cl.35, PRO2286, CSDD2, FLJ16517

WB

51972214

Liquid