LIX1L Antilichaam / Limb en CZS uitgedrukt eiwit 1-achtig

ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Western Blot: 0.25-0.5 µg/mL

Q8IVB5

Human, Mouse, Rat

E.coli-derived human LIX1L recombinant protein (Position: L77-H333) was used as the immunogen for the LIX1L antibody.

ELISA, FACS, WB

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This LIX1L antibody is available for research use only.

After reconstitution, the LIX1L antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the LIX1L antibody should be determined by the researcher.

Western blot analysis of LIX1L using anti-LIX1L antibody. Lane 1: rat skeletal muscle tissue lysates, Lane 2: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIX1L antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for LIX1L at approximately 37 kDa. The expected molecular weight of LIX1L is ~37 kDa.