Low Sample Volume ELISA Kit voor Amylin

IAPP; IAP; DAP; Islet Amyloid Polypeptide; Insulinoma Amyloid Polypeptide; Diabetes-associated peptide

Enzyme-linked immunosorbent assay for Antigen Detection.

Metabolic pathway; Endocrinology; Hepatology

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Amylin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Amylin and unlabeled Mini Samples Amylin (Standards or samples) with the pre-coated antibody specific to Mini Samples Amylin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Amylin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Amylin in the sample.

3h

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

12.35-1,000pg/mL

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Mini Samples Amylin were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Mini Samples Amylin were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV

The minimum detectable dose of this kit is typically less than 4.37pg/mL

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

48T, 96T, 96Tx5, 96Tx10, 96Tx100

This assay has high sensitivity and excellent specificity for detection of Mini Samples Amylin. No significant cross-reactivity or interference between Mini Samples Amylin and analogues was observed.

Amylin

Competitive Inhibition

Rattus norvegicus (Rat)