LRRC71 Antilichaam/Leucinerijke repeterende proteïne 71

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

Q8N4P6

Human, Mouse, Rat

E.coli-derived human C1orf92/LRRC71 recombinant protein (Position: K35-D543) was used as the immunogen for the LRRC71 antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This LRRC71 antibody is available for research use only.

After reconstitution, the LRRC71 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the LRRC71 antibody should be determined by the researcher.

Western blot analysis of C1orf92/LRRC71 using anti-LRRC71 antibody. Lane 1: human PC-3 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC71 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. LRRC71 (~62 kDa predicted) was detected as a major band at ~62 kDa, with a weaker band at ~70 kDa consistent with differential glycosylation or isoform expression. Additional lower bands (~22 kDa) observed in rodent tissues likely represent truncated or species-specific LRRC71 forms, as reported for related LRR-family proteins.