LSD1 Antilichaam

LSD1 Antibody: Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription. LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation. Experiments have shown that CoREST, a SANT domain-containing corepressor acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein, inhibits CoREST/LSD1 activity in vitro. LSD1-mediated histone demethylation thus may have significant effects on gene expression.

23028

O60341

NP_001009999

Human, Mouse, Rat

3761

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 2) shows similar LSD1 expression profile in human and mouse cell lines detected by two independent anti-LSD1 antibodies that recognize different epitopes, 3761 against N-terminus domain and 14-050 against recombinant fusion protein. LSD1 proteins are detected in the most tested cell lines that include mouse (3T3/NIH) and rat cell lines (YB2/0) at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3761 14-050

Homeostasis

LSD1 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1286 - P815 Cell Lysate

1 mg/mL

LSD1 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

LSD1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human LSD1 has 2 isoforms, including isoform 1 (852aa, 93kD) and isoform 2 (876aa, 95kD) . Mouse LSD1 has one isoform (853aa, 93kD) and Rat LSD1 also has one isoform (872aa, 95kD) . 3761 can detect all isoforms of human, mouse and rat.

KDM1

Cat. No. 1224 - Daudi Cell Lysate

Cat. No. 1205 - Jurkat Cell Lysate

Cat. No. 1211 – HepG2 Cell Lysate

Lysine (K) -specific demethylase 1

Homo sapiens

Shi et al. Cell 2004; 119:941-53.

Kouzarides et al. Curr. Opin. Genet. Dev. 2002; 12:198-209.

Shi et al. Mol. Cell 2005; 19:857-64.

Nakamura et al. Mol. Cell 2002; 10:1119-28.

LSD1 Antibody: AOF2, KDM1, LSD1, BHC110, AOF2, KIAA0601, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110

ELISA, WB, IHC-P, IF

58761544

Liquid