LSD1 Antilichaam

Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction (1) . Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription (2) . LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation (3,4) . Experiments have shown that CoREST, a SANT domain-containing corepressor (5) acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein (6), inhibits CoREST/LSD1 activity in vitro (3) . LSD1-mediated histone demethylation thus may have significant effects on gene expression.

23028

O60341

NP_001009999

Human, Mouse, Rat

8223

Unconjugated

Primary Antibodies

Homeostasis

LSD1 antibody is affinity chromatography purified via peptide column.

Cat. No. 1203 - A549 Cell Lysate

1 mg/mL

LSD1 antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

LSD1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

IgG

At least two isoforms of LSD1 are known to exist; this antibody detects both.

Human LSD1 has 2 isoforms, including isoform 1 (852aa, 93kD) and isoform 2 (876aa, 95kD) . Mouse LSD1 has one isoform (853aa, 93kD) and Rat LSD1 also has one isoform (872aa, 95kD) . 8223 can detect LSD1 in human, mouse and rat.

KDM1A

Cat. No. 10-901 - Human Spleen Tissue Slide

Lysine (K) -specific demethylase 1 A

Homo sapiens

Shi et al. Cell 2004; 119:941-53.

Kouzarides et al. Curr. Opin. Genet. Dev. 2002; 12:198-209.

Shi et al. Mol. Cell 2005; 19:857-64.

Nakamura et al. Mol. Cell 2002; 10:1119-28.

Lysine-specific histone demethylase, amine oxidase flavin containing domain protein 2, AOF2

ELISA, WB, IHC-P

58761544

Liquid