LTBR Antilichaam/Lymptotoxine-beta-receptor

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

P36941

Human

E.coli-derived human LTBR recombinant protein (Position: Q31-Q404) was used as the immunogen for the LTBR antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This LTBR antibody is available for research use only.

After reconstitution, the LTBR antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the LTBR antibody should be determined by the researcher.

Western blot analysis of LTBR using anti-LTBR antibody. Lane 1: human Hela whole cell lysates, Lane 2: human Hut-78 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LTBR antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot detection of LTBR shows a band at 55-65 kDa across multiple human cell lines. Although the calculated mass is ~47 kDa, the receptor commonly migrates larger due to extensive N-glycosylation in the ectodomain. No band is observed in Hut-78 cell lysate, consistent with low/absent LTBR expression in T-lineage cells.