Luciferase Polyklonale Antilichaam, Cy3 Vervoeging

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Luciferin 4-monooxygenase

7100

Cytoplasm

101-220/550

Luciferase

Cy3

KLH conjugated synthetic peptide derived from Firefly Luciferase

IF (IHC-P), IF (IHC-F), IF (ICC)

Purified by Protein A.

512,550nm/570,615nm

1µg/µl

IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Unmodified

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

7100

Firefly

Photinus pyralis (Common eastern firefly)