Lysosoom Green Probes

Green Fluorescent acidotropic probes for labeling and tracking acidic organelles in live cells

IF

Liquid

Store at -20 °C in the dark for 6 months.

The Lysosome Green Probes, which consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH, are freely permeant to cell membranes and typically concentrate in spherical organelles. Their mechanism of retention has not been firmly established but is likely to involve protonation and retention in the membranes of the organelles, although staining is generally not reversed by subsequent treatment of the cells with weakly basic cell-permeant compounds. Note that in Lysosome Green Probes stained cells, the lysosomal fluorescence may constitute only a small portion of total cellular fluorescence, making it difficult to quantitate the number of lysosomes by flow cytometry or fluorometry.

Weakly basic amines selectively accumulate in cellular compartments with low internal pH and can be used to investigate the biosynthesis and pathogenesis of lysosomes. The Lysosome Green Probes are fluorescent acidotropic probes for labeling and tracking acidic organelles in live cells. These probes have several important features, including high selectivity for acidic organelles and effective labeling of live cells at nanomolar concentrations.

Ex/Em = 504/511 nm