MADD Antilichaam

MADD Antibody: MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1) associated protein though their death domains. Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2. MADD shares 98% identity with DENN (for differentially expressed in neoplastic vs. normal cells), which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3) . MADD has greater than 94% overall identity to a GDP/GTP exchange protein Rab3-GEP. MADD is 87% identical to KIAA0358, a brain protein of unknown function. Identification of MADD as a component of the TNFR1 signaling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein.

8567

Q8WXG6

AAD12154

Human, Mouse, Rat

Unconjugated

Primary Antibodies

Signal Transduction

MADD Antibody is DEAE purified.

Cat. No. 1201 - HeLa Cell Lysate

1 mg/ml

MADD Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

MADD antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

200 to 220 kDa

IgG

MADD

Cat. No. 1212 - 3T3 Cell Lysate

Cat. No. 10-901 - Human Spleen Tissue Slide

Cat. No. 17-001 - HeLa Cell Slide

MAP-kinase activating death domain

Homo sapiens

Schievella AR, Chen JH, Graham JR, Lin LL. MADD, a novel death domain protein that interacts with the type 1 tumor necrosis factor receptor and activates mitogen-activated protein kinase. J Biol Chem 1997;272:12069-12075

Chow VT, Lee SS. DENN, a novel human gene differentially expressed in normal and neoplastic cells. DNA Seq 1996;6:263-273

Zhang Y, Zhou L, Miller CA. A splicing variant of a death domain protein that is regulated by a mitogen-activated kinase is a substrate for c-Jun N-terminal kinase in the human central nervous system. Proc Natl Acad Sci U S A 1998;95:2586-2591

Brown TL and Howe PH. MADD is highly homologous to a Rab3 guanine-nucleotide exchange protein (Rab3-GEP) . Curr Biol 1998;8:R191

MADD Antibody: DENN, IG20, RAB3GEP, DENN, KIAA0358, MAP kinase-activating death domain protein, Differentially expressed in normal and neoplastic cells

ELISA, WB, ICC, IF

3289973

Liquid