MAP2K1 Antilichaam / Mitogeen-geactiveerd eiwit kinase 1

ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Western Blot: 0.25-0.5 µg/mL

Q02750

Human, Mouse, Rat

E.coli-derived human MEK1/MAP2K1 recombinant protein (Position: M1-V393) was used as the immunogen for the MAP2K1 antibody.

ELISA, FACS, IF, IHC, ICC, WB

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This MAP2K1 antibody is available for research use only.

After reconstitution, the MAP2K1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the MAP2K1 antibody should be determined by the researcher.

Cytoplasmic, Nuclear

Western blot analysis of MAP2K1 using anti-MAP2K1 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse NH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substratewith Tanon 5200 system. A specific band was detected for MAP2K1 at approximately 45 kDa. The expected molecular weight of MAP2K1 is ~45 kDa.