MAP2K1 cDNA

MAP2K1 is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Mitogen-activated protein kinase kinase 1, MAP2K1, CFC3, MAP kinase kinase 1, MAPKK1, MEK1, MKK1, PRKMK1, ERK activator kinase 1, MAPK/ERK kinase 1

15q22.1-q22.33

Human

PATGen (puc19-derived cloning vector)

ATGCCCAAGA AGAAGCCGAC GCCCATCCAG CTGAACCCGG CCCCCGACGG CTCTGCAGTT AACGGGACCA GCTCTGCGGA GACCAACTTG GAGGCCTTGC AGAAGAAGCT GGAGGAGCTA GAGCTTGATG AGCAGCAGCG AAAGCGCCTT GAGGCCTTTC TTACCCAGAA GCAGAAGGTG GGAGAACTGA AGGATGACGA CTTTGAGAAG ATCAGTGAGC TGGGGGCTGG CAATGGCGGT GTGGTGTTCA AGGTCTCCCA CAAGCCTTCT GGCCTGGTCA TGGCCAGAAA GCTAATTCAT CTGGAGATCA AACCCGCAAT CCGGAACCAG ATCATAAGGG AGCTGCAGGT TCTGCATGAG TGCAACTCTC CGTACATCGT GGGCTTCTAT GGTGCGTTCT ACAGCGATGG CGAGATCAGT ATCTGCATGG AGCACATGGA TGGAGGTTCT CTGGATCAAG TCCTGAAGAA AGCTGGAAGA ATTCCTGAAC AAATTTTAGG AAAAGTTAGC ATTGCTGTAA TAAAAGGCCT GACATATCTG AGGGAGAAGC ACAAGATCAT GCACAGAGAT GTCAAGCCCT CCAACATCCT AGTCAACTCC CGTGGGGAGA TCAAGCTCTG TGACTTTGGG GTCAGCGGGC AGCTCATCGA CTCCATGGCC AACTCCTTCG TGGGCACAAG GTCCTACATG TCGCCAGAAA GACTCCAGGG GACTCATTAC TCTGTGCAGT CAGACATCTG GAGCATGGGA CTGTCTCTGG TAGAGATGGC GGTTGGGAGG TATCCCATCC CTCCTCCAGA TGCCAAGGAG CTGGAGCTGA TGTTTGGGTG CCAGGTGGAA GGAGATGCGG CTGAGACCCC ACCCAGGCCA AGGACCCCCG GGAGGCCCCT TAGCTCATAC GGAATGGACA GCCGACCTCC CATGGCAATT TTTGAGTTGT TGGATTACAT AGTCAACGAG CCTCCTCCAA AACTGCCCAG TGGAGTGTTC AGTCTGGAAT TTCAAGATTT TGTGAATAAA TGCTTAATAA AAAACCCCGC AGAGAGAGCA GATTTGAAGC AACTCATGGT TCATGCTTTT ATCAAGAGAT CTGATGCTGA GGAAGTGGAT TTTGCAGGTT GGCTCTGCTC CACCATCGGC CTTAACCAGC CCAGCACACC AACCCATGCT GCTGGCGTCT AA

MAP2K1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, MAP2K1, ATGD0015-10 µg, ATGD0015-20 µg, ATGD0015-50 µg, ATGD0015-100 µg, ATGD0015-250 µg, ATGD0015-500 µg, ATGD0015-1 mg, ATGD0015-10, ATGD0015-20, ATGD0015-50, ATGD0015-100, ATGD0015-250, ATGD0015-500, ATGD0015-1

Store the plasmid at -20C.

Lyophilized

Signal Transduction

176872

NP_002746.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_002755.3

1182bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.