MAPK14 Antilichaam / Mitogen-geactiveerd eiwit kinase 14 / p38 alfa

Flow cytometry: 1-3ug/million cells, Immunoprecipitation: 2-4ug/500ug of lysate, Immunohistochemistry: 2-5 µg/mL, Western Blot: 0.25-0.5 µg/mL

Q16539

Human, Mouse, Rat

A synthetic peptide corresponding to a sequence at the C-terminus of human p38 alpha/MAPK14 was used as the immunogen for the MAPK14 antibody.

FACS, IP, IHC, WB

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This MAPK14 antibody is available for research use only.

After reconstitution, the MAPK14 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the MAPK14 antibody should be determined by the researcher.

Cytoplasmic, Nuclear

Immunohistochemical staining of MAPK14 using anti- MAPK14 antibody. MAPK14 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti- MAPK14 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.