McCC2 (NM 022132) Humane overexpressie Lysaat

Transient overexpression lysate of methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (MCCC2), nuclear gene encoding mitochondrial protein

MCCB; MCCCbeta

64087

NM_022132

5q13.2

HEK293T

C-Myc/DDK

Metabolic pathways, Valine, leucine and isoleucine degradation

Metabolic pathways, Valine, leucine and isoleucine degradation

Liquid

Druggable Genome

61.3 kDa

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

MCCC2

Human

NP_071415

HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.

https://www.ncbi.nlm.nih.gov/gene/64087

https://www.ncbi.nlm.nih.gov/nuccore/NM_022132

https://www.ncbi.nlm.nih.gov/nuccore/NP_071415