MCEMP1 Antilichaam / Mast cel-uitgedrukt membraaneiwit 1

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

Q8IX19

Human, Mouse, Rat

E.coli-derived human MCEMP1 recombinant protein (Position: M1-Q183) was used as the immunogen for the MCEMP1 antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This MCEMP1 antibody is available for research use only.

After reconstitution, the MCEMP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the MCEMP1 antibody should be determined by the researcher.

Western blot analysis of MCEMP1 using anti-MCEMP1 antibody. Lane 1: human THP-1 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse Hepa1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCEMP1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. MCEMP1 antibody detects a single band at ~28 kDa across the indicated samples. Although the calculated mass is ~21 kDa, MCEMP1 is a glycosylated, single-pass membrane protein that commonly migrates at ~27-30 kDa on SDS-PAGE.