MCF-7 Lysaat

MCF-7 cells are breast cancer cell line isolated in 1970 from a 69 year old woman, in 1970s. MCF-7 cells are a well characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin, endothelin, GFAP, neurofilament, vimentin.

Human

WB

2 mg/mL (determined by BCA assay)

MCF7, ATGL0009-10 µg, ATGL0009-20 µg, ATGL0009-50 µg, ATGL0009-100 µg, ATGL0009-250 µg, ATGL0009-500 µg, ATGL0009-1 mg, ATGL0009-10, ATGL0009-20, ATGL0009-50, ATGL0009-100, ATGL0009-250, ATGL0009-500, ATGL0009-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : MCF-7 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody