MCM3AP Antilichaam / Minichromosoom onderhoudscomplex-bindend eiwit 3-geassocieerd eiwit

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

O60318

Human, Mouse, Rat

E.coli-derived human GANP/MCM3AP recombinant protein (Position: R1275-H1715) was used as the immunogen for the MCM3AP antibody.

WB, ICC, IF, FACS, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This MCM3AP antibody is available for research use only.

After reconstitution, the MCM3AP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the MCM3AP antibody should be determined by the researcher.

Nuclear, cytoplasmic

Immunofluorescent staining of GANP/MCM3AP using anti-MCM3AP antibody (green) and anti-Beta Tubulin antibody (red) . GANP/MCM3AP was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-MCM3AP antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.