MCMBP Antilichaam/MCM-bindend eiwit

Western Blot: 0.25-0.5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, ELISA: 0.1-0.5 µg/mL

Q9BTE3

Human, Mouse, Rat

E.coli-derived human MCMBP recombinant protein (Position: E234-E534) was used as the immunogen for the MCMBP antibody.

WB, IP, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This MCMBP antibody is available for research use only.

After reconstitution, the MCMBP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the MCMBP antibody should be determined by the researcher.

Immunoprecipitation of MCMBP in 293T whole cell lysate. Western blot analysis of MCMBP using anti-MCMBP antibody. Lane 1: 293T whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-MCMBP antibody in 293T whole cell lysate, Lane 3: anti-MCMBP antibody (2ug) + 293T whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MCMBP antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. The expected molecular weight of MCMBP is ~73 kDa.