MDA-MB-231 Lysaat

MDA-MB-231 cell line was metastatic human breast cancer cell line originally isolated from pleural effusions of a breast cancer patient in the early 1970s. MDA-MB-231 cells possess epithelial-like morphology and exhibit invasive properties when cultured in vitro and when transplanted into mice. MDA-MB-231 is widely used as a model for estrogen receptor negative breast cancer with applications in the study of tumorigenicity, metastasis, and cell invasion.

Human

WB

2 mg/mL (determined by BCA assay)

MDA-MB231, ATGL0035-10 µg, ATGL0035-20 µg, ATGL0035-50 µg, ATGL0035-100 µg, ATGL0035-250 µg, ATGL0035-500 µg, ATGL0035-1 mg, ATGL0035-10, ATGL0035-20, ATGL0035-50, ATGL0035-100, ATGL0035-250, ATGL0035-500, ATGL0035-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : MDA-MB-231 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody