MDM2 Antilichaam

MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 . Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 . Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 .

4193

Q00987

NP_002383.2

Human

MDM2 antibody was raised against a peptide sequence around aa.18~22 (Q-I-P-A-S) derived from Human MDM2. .

Unconjugated

Primary Antibodies

Other

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

None

Blue Ice

Store antibody at -20˚ C for up to one year.

90 kDa

This antibody detects endogenous levels of total MDM2 protein.

Western Blot: 1:500~1:1000

MDM2

MDM2 oncogene, E3 ubiquitin protein ligase

Homo sapiens

Haupt, Y. et al. (1997) Nature 387, 296-299. Mayo, L. D. and Donner, D. B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603. Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981. Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.

HDMX, hdm2, ACTFS, CCN5, CT58, CTGFL, UNQ228/PRO261, HDMX

WB

89993689

Liquid