MED21 cDNA

MED21 is a member of the mediator complex subunit 21 family. The encoded protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants.

HSrb7, SRB7, SURB7

12p11.23

Human

PATGen (puc19-derived cloning vector)

ATGGCGGATCGGCTCACGCAGCTTCAGGACGCTGTGAATTCGCTTGCAGATCAGTTTTGTAATGCCATTGGAGTATTGCAGCAATGTGGTCCTCCTGCCTCTTTCAATAATATTCAGACAGCAATTAACAAAGACCAGCCAGCTAACCCTACAGAAGAGTATGCCCAGCTTTTTGCAGCACTGATTGCACGAACAGCAAAAGACATTGATGTTTTGATAGATTCCTTACCCAGTGAAGAATCTACAGCTGCTTTACAGGCTGCTAGCTTGTATAAGCTAGAAGAAGAAAACCATGAAGCTGCTACATGTCTGGAGGATGTTGTTTATCGAGGAGACATGCTTCTGGAGAAGATACAAAGCGCACTTGCTGATATTGCACAGTCACAGCTGAAGACAAGAAGTGGTACCCATAGCCAGTCTCTTCCAGACTCATAG

MED21, hSrb7, SRB7, SURB7, MED21, ATGD0476-10 µg, ATGD0476-20 µg, ATGD0476-50 µg, ATGD0476-100 µg, ATGD0476-250 µg, ATGD0476-500 µg, ATGD0476-1 mg, ATGD0476-10, ATGD0476-20, ATGD0476-50, ATGD0476-100, ATGD0476-250, ATGD0476-500, ATGD0476-1

Store the plasmid at -20C.

Lyophilized

Epigenomics (Transcription & Translation)

603800

NP_004255.2

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_004264.4

435bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.