MED28 Antilichaam

MED28 Antibody: The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED28, also known as Magicin. MED28 is expressed in many cell lines and tissues. It has been shown that a down-regulation of MED28 expression in NIH3T3 cells results in a significant induction of several genes associated with smooth muscle cell differentiation and conversely its over-expression represses expression of SMC genes. MED28 can also form a ternary complex with Grb2 and Merlin, the neurofibromatosis 2 tumor suppressor protein, indicating that MED28 may play a role in Merlin's tumor suppressive activity. MED28 has also been recently identified as an HIV dependency factor (HDF), suggesting that MED28 may be an important drug target in HIV treatment. At least two isoforms of MED28 are known to exist.

80306

Q9H204

NP_079481

Human, Mouse

Unconjugated

Primary Antibodies

Infectious Disease

MED28 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1303 - Human Brain Tissue Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Rat: (100%), Bovine: (100%)

MED28 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

MED28 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

MED28

Cat. No. 10-301 - Human Brain Tissue Slide

Mediator complex subunit 28

Homo sapiens

Sato S, Tomomori-Sato C, Parmely TJ, et al. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Mol. Cell2004; 14:685-91.

Beyer KS, Beauchamp RL, Lee MF, et al. Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation. J. Biol. Chem.2007; 282:32152-7.

Wiederhold T, Lee MF, James M, et al. Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Oncogene2004; 23:8815-25.

Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.

MED28 Antibody: EG1, magicin, 1500003D12Rik, EG1, FKSG20, Mediator of RNA polymerase II transcription subunit 28, Endothelial-derived protein 1, Magicin

ELISA, WB, IHC-P

52851391

Liquid