MELTF Antilichaam / Melanotransferrine / MFI2

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, ELISA: 0.1-0.5 µg/mL

P08582

Human, Mouse, Rat

E.coli-derived human MFI2/MELTF recombinant protein (Position: A60-D647) was used as the immunogen for the MELTF antibody.

WB, IHC, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This MELTF antibody is available for research use only.

After reconstitution, the MELTF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the MELTF antibody should be determined by the researcher.

Western blot analysis of MELTF using anti-MELTF antibody. Lane 1: rat skin tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse skin tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MELTF antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A single band is detected at ~90 kDa in rat and mouse samples, running above the ~80 kDa prediction. The higher migration is consistent with the mature, heavily N-glycosylated and GPI-anchored forms of melanotransferrin commonly observed in rodent tissues.