Menselijk EDAR antilichaam

Tumor necrosis factor receptor superfamily member EDAR is a protein that in humans is encoded by the EDAR gene. EDAR and other genes provide instructions for making proteins that work together during embryonic development. These proteins form part of a signaling pathway that is critical for the interaction between two cell layers, the ectoderm and the mesoderm. In the early embryo, these cell layers form the basis for many of the body's organs and tissues. Ectoderm-mesoderm interactions are essential for the proper formation of several structures that arise from the ectoderm, including the skin, hair, nails, teeth, and sweat glands.

Tumor necrosis factor receptor superfamily member EDAR, DL, ECTD10A, ECTD10B, ED1R, ED3, ED5, EDA-A1R, EDA1R, EDA3, HRM1

Human

Human

Recombinant human EDAR (27-448aa) purified from E.coli

AT19E8

Unconjugated

ELISA, WB, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

EDAR, ATGA0334-10 µg, ATGA0334-20 µg, ATGA0334-50 µg, ATGA0334-100 µg, ATGA0334-250 µg, ATGA0334-500 µg, ATGA0334-1 mg, ATGA0334-10, ATGA0334-20, ATGA0334-50, ATGA0334-100, ATGA0334-250, ATGA0334-500, ATGA0334-1

Monreal AW, et al. (1999) Nat Genet 22 (4) :366-9.; ; Aswegan AL, et al. (1997) Am J Med Genet 72 (4) :462-7.; ; Entrez Gene: EDAR ectodysplasin A receptor.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_071731

Q9UNE0

The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EDAR antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : Recombinant protein EDAR ; ; Lane 2 : A549 cell lysate ; ; Lane 3 : Ramos cell lysate

Flow cytometry analysis of EDAR in HeLa cells. The cell was stained with ATGA0334 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray)