MERS-CoV Spike antilichaam

ELISA: MERS Spike Antibody (1 µg/mL) specifically recognizes MERS Spike and Spike S1 recombinant protein, but not interacted MERS Spike S2 and RBD recombinant protein in ELISA.{ATGA0598-Addpic.jpg}

MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses. MERS-CoV was first identified in the Kingdom of Saudi Arabia in 2012, which is a single and positive stranded RNA virus. Dromedary camels are widely considered as the source of the transmission of MERS-CoV. The rate of human transmission among household contacts of MERS patients has been approximately 5 % based on serological analysis. MERS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein, responsible for allowing the virus to attach to and fuse with the membrane of a host cell and is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. MERS-CoV S mediates viral attachment and fusion to human cells via human cellular receptor DPP4, also known as CD26. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Middle East respiratory syndrome coronavirus, Human betacoronavirus 2c EMC/2012, MERS-CoV, MERS, MERSCoV SP, Spike glycoprotein, S glycoprotein, S, Spike protein

MERS-CoV

MERS-CoV

Recombinant MERS-CoV Spike (18-1296aa) purified from Baculovirus

AT6E6

Unconjugated

ELISA

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Covid-19, covid19, sars, sars2, ATGA0598-10 µg, ATGA0598-20 µg, ATGA0598-50 µg, ATGA0598-100 µg, ATGA0598-250 µg, ATGA0598-500 µg, ATGA0598-1 mg, ATGA0598-10, ATGA0598-20, ATGA0598-50, ATGA0598-100, ATGA0598-250, ATGA0598-500, ATGA0598-1

Junghyun Goo., et al. (2020) Virus Res. 278:197863. ; ; Yan-Hua Li., et al. (2019) Engineering. 5:940-947.; ; Lingshu Wang., et al. (2018) J Virol. 92:e02002-2017. ; ; Nicolas Papageorgiou., et al. (2016) Acta Crystallogr D Struct Biol. 72:192-202.; ; Xiao-Yan Che., et al. (2004) J Clin Microbiol. 42:2629-2635.;

Lee K, et al. Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus. (Microbiol Immunol. 2018) {https://pubmed.ncbi.nlm.nih.gov/30117617/}; ; Goo J, et al. Characterization of novel monoclonal antibodies against MERS-coronavirus spike protein. (Virus Res. 2020) {https://pubmed.ncbi.nlm.nih.gov/31945421/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Virus

AFS88936

K0BRG7