Mesotheline Antilichaam / MSLN

Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Western blot: 1-2 µg/mL

Q13421

Human

A portion of amino acids 273-407 from human Mesothelin protein was used as the immunogen for the Mesothelin antibody.

MSLN/3383

WB

Protein G affinity

Purified

This Mesothelin antibody is available for research use only.

Aliquot the Mesothelin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the Mesothelin antibody should be determined by the researcher.

Cell membrane, Golgi apparatus, Secreted

Western blot testing of human OVCAR-3 cell lysate with Mesothelin antibody (clone MSLN/3383) . Predicted molecular weight ~69 kDa (precursor), ~40 kDa (cleaved form) . Glycosylation may make the protein run higher than expected.