MEST Antilichaam / Mesoderm-specifiek transcripteiwit

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, ELISA: 0.1-0.5 µg/mL

Q5EB52

Human, Mouse, Rat

E.coli-derived human MEST recombinant protein (Position: A21-D320) was used as the immunogen for the MEST antibody.

WB, IHC, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This MEST antibody is available for research use only.

After reconstitution, the MEST antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the MEST antibody should be determined by the researcher.

Immunohistochemical staining of MEST using anti-MEST antibody. MEST was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MEST antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.