MET-eiwit (C-His, mens)

MET

AUTS9, c-Met, DA11, DFNB97, HGFR, RCCP2

C-His

< 1.0 EU per μg of the protein as determined by the LAL method

≥ 90 % as determined by SDS-PAGE.

1.Flow cytometric analysis of anti-c-MET CAR expression. 5e5 of anti-c-MET CAR-293 cells were stained with 100 μL of 20μg/ml of AF 488-conjugated human HGFR Protein (Site-Specific AF 488 Conjugation, ECD, His Tag, Cat. No. 10692-H86H-SD) and negative control protein. Non-transduced 293 cells were used as a control (left) . AF 488 signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of AF 488-conjugated c-MET protein to PBMC cells. PBMC cells were stained with PE conjugated anti-CD3 antibody and AF 488-conjugated human HGFR Protein (Site-Specific AF 488 Conjugation, ECD, His Tag, Cat. No. 10692-H86H-SD) and detected by flow cytometry. PE signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 488 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) .

Met1-Thr932

The recombinant Human MET consists of AA Met1-Thr932 with a polyhistidine tag at the C-terminus. It has a predicted molecular mass of 105.1 kDa.

Human