MET-eiwit (C-human IgG1-Fc, muis)

MET

C-Met, HGF, HGFR, Met, Par4

C-human IgG1-Fc

< 1.0 EU per μg of the protein as determined by the LAL method

≥ 92 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

1.Immobilized Recombinant Mouse HGF / Hepatocyte Growth Factor Protein (His Tag) (Cat: 50038-M08H) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse c-MET / HGFR Protein (Fc Tag) (Cat: 50622-M02H), the EC50 is 7.5-22.5 ng/mL (QC tested) . 2.Immobilized Recombinant Human HGF Protein (Cat: 10463-HNAS) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse c-MET / HGFR Protein (Fc Tag) (Cat: 50622-M02H), the EC50 is 1.5-4.5 ng/mL (Routinely tested) .

Met1-Asn929

The recombinant mouse Met/Fc chimera is a disulfide-linked homodimer of the Met which is a heterodimer composed of the proteolytically cleaved α and β subunits. Each α and β together with the C-terminal Fc tag consists of 1146 amino acids and has a predicted molecular mass of 128 (α =32 + Fc tagged β=96) kDa. The apparent molecular mass of the rm MET/Fc heterodimer thus is approximately 43 kDa and 115-120 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.

Mouse