MGARP Antilichaam / Mitochondriën-gelokaliseerd glutaminezuurrijk eiwit

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

Q8TDB4

Human, Mouse, Rat

E.coli-derived human C4orf49/MGARP recombinant protein (Position: M1-E155) was used as the immunogen for the MGARP antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This MGARP antibody is available for research use only.

After reconstitution, the MGARP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the MGARP antibody should be determined by the researcher.

Western blot analysis of C4orf49/MGARP using anti-MGARP antibody. Lane 1: human whole cell lysates, Lane 2: human whole cell lysates, Lane 3: human SK-OV-3 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGARP antibody at 0.25 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. MGARP (~25 kDa predicted) was detected at ~38 kDa, consistent with anomalously slow SDS-PAGE migration of this glutamic-acid–rich, intrinsically disordered mitochondrial transmembrane protein.