MIB2 Antilichaam / Mindbomb 2

Beyond canonical Notch signaling, MIB2 has been implicated in cytoskeletal organization and broader ubiquitin-mediated quality control. Altered MIB2 expression or activity can influence cell fate decisions, neural and immune system function, and tumor biology, where disrupted ligand trafficking and signaling balance contribute to disease phenotypes. Its functional overlap and divergence relative to the paralog MIB1 highlight MIB2 as a distinct regulator with tissue- and context-specific roles. The MIB2 antibody enables specific detection of endogenous MIB2 in cells and tissues for applications including western blot, immunofluorescence, and immunohistochemistry. Researchers use the MIB2 antibody to quantify expression, assess subcellular localization at membranes and endosomes, and monitor pathway modulation under genetic or pharmacologic perturbations. With high specificity and consistent performance, the MIB2 antibody supports studies of ubiquitin signaling, Notch pathway regulation, and disease mechanisms.

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3 µg/mL, Immunoprecipitation: 2ug per 500ug of lysate

Q96AX9

Human, Mouse, Rat

Recombinant human protein (amino acids A71-I572) was used as the immunogen for the MIB2 antibody.

WB, FACS, IP

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This MIB2 antibody is available for research use only.

After reconstitution, the MIB2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the MIB2 antibody should be determined by the researcher.

Cytoplasm

Flow cytometry testing of fixed and permeabilized human SiHa cells with MIB2 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= MIB2 antibody.